Biotechnological production of microbial oils from carbon dioxide using microalgae and oleaginous yeasts in an integrated process

Ayşe Koruyucu, doctoral thesis Technical University of Munich, 2024

Bioprocess engineering tools enabled the production of Microchloropsis salina biomass in open thin-layer cascade photobioreactors with complete carbon dioxide fixation. Biomass separation, homogenization and enzymatic hydrolysis allowed a partial saccharification of the microalgae biomass. Utilizing a membrane bioreactor with total cell retention, after phosphate depletion, the production of yeast oil in Cutaneotrichosporon oleaginosus was possible despite the dilute sugar hydrolysate.

Publications

• Koruyucu A, Peest T, Korzin E, Gröninger L, Patricia, Brück T, Weuster-Botz D (2024): Cell disruption and hydrolysis of Microchloropsis salina biomass as a feedstock for fermentation. Appl Sci 14: 9667.
• Koruyucu A, Schädler T, Gniffke A, Mundt K, Krippendorf S, Urban P, Blums K, Halim B, Brück T, Weuster-Botz D (2024): Energy-efficient production of Microchloropsis salina biomass with high CO₂ fixation yield in open thin-layer cascade photobioreactors. Processes 12: 1303.
• Koruyucu A, Blums K, Peest T, Schmack-Rauscher L, Brück T, Weuster-Botz D (2023): High-cell-density yeast oil production with diluted substrates imitating microalgae hydrolysate using a membrane bioreactor. Energies 16: 1757.