Characterization of anodic respiration by Pseudomonas putida in stirred-tank bioreactors

Sarah Hintermayer, Doctoral Thesis Technical University of Munich, 2016

Anodic respiration could represent a possibility to selectively affect product formation by microorganisms. A stirred-tank bioreactor was modified so that sufficient convective mass transport in mediated anodic respiration could be achieved with a recombinant Pseudomonas putida. By controlling the redox potential in the medium, the production of para-hydroxybenzoic acid from citric acid could be increased by 70 % compared to aerobic respiration with Pseudomonas putida.

 

Publications

• Hintermayer S, Weuster-Botz D (2017): Experimental validation of in silico estimated biomass yields of Pseudomonas putida KT2440. Biotechnol J  12: 1600720.
• Hintermayer S, Yu S, Kroemer JO, Weuster-Botz D (2016): Anodic respiration of Pseudomonas putida KT2440 in a stirred-tank bioreactor. Biochem Eng J 115: 1-13.