The co-cultivation of the two fungi Aspergillus niger and Trichoderma reesei in stirred tank bioreactors enabled the efficient production of an enzyme cocktail for the saccharification of wheat bran. Combining enzyme production with subsequent hydrolysis in a one-pot process meant no need for the complex separation and purification of the enzymes. Switching off the gassing and increasing the temperature was sufficient to prevent the metabolism of the released sugars during hydrolysis.
Publications
- Mittermeier F, Fischer F, Hauke S, Hirschmann P, Weuster-Botz D (2024): Valorization of wheat bran by co-cultivation of fungi with integrated hydrolysis to provide sugars and animal feed. BioTech 13: 15.
- Mittermeier F, Hafner N, Xypolia Vasila K, Weuster-Botz D (2023): Co-cultivation of Aspergillus niger and Trichoderma reesei enables efficient production of enzymes for the hydrolysis of wheat bran. Chem Ing Tech 95: 565-575.
- Mittermeier F, Bäumler M, Arulrajah P, García Lima JJ, Hauke S, Stock A, Weuster-Botz D (2023): Artificial microbial consortia for bioproduction processes. Eng Life Sci 23: e2100152.