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Technical University of Munich

In-vivo Metabolic Control Analysis for the improvement of the L-cysteine production with Escherichia coli

Daniel Caballero, doctoral thesis Technical University of Munich, 2024

In-vivo Metabolic Control Analysis was used to identify bottlenecks in the L-cysteine biosynthetic pathway of engineered Escherichia coli producer cells from a fed-batch production process. Two L-cysteine synthases were identified as rate limiting for the L-cysteine production. Mutant strains with additionally overexpressed L-cysteine synthases demonstrated an increase in the maximal L-cysteine concentration of up to 47%, and in the L-cysteine productivity of more than 120% in the production process.

Publications

  • Caballero Cerbon DA, Gebhard L, Dokuyucu R, Ertl T, Härtl S, Mazhar A, Weuster-Botz D (2024): Challenges and advances in the bioproduction of L-cysteine. Molecules 29: 486.
  • Caballero Cerbon DA, Widmann J, Weuster-Botz D (2024): Metabolic control analysis enabled the improvement of a L-cysteine production process with Escherichia coli. Appl Microbiol Biotechnol 108: 108.
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