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Technical University of Munich

L-phenylalanine production with Escherichia coli reporter strains in a novel two-compartment bioreactor

Manh Dat Hoang, doctoral thesis Technical University of Munich, 2024

The application of a stirred-tank bioreactor with a ‘coiled flow inverter’ as a bypass revealed the effects of dynamic environmental conditions during the L-phenylalanine production with E. coli reporter strains, whose reporter proteins were monitored by flow cytometry. Fed-batch processes with increasing hydraulic residence times in the bypass led to a decreased cell growth and product formation. However, the level of population heterogeneity was reduced for selected cellular characteristics.

Publications
  • Hoang MD, Polte I, Frantzmann L, von den Eichen N, Heins AL, Weuster-Botz D (2023): Impact of mixing insufficiencies on L-phenylalanine production with an Escherichia coli reporter strain in a novel two-compartment bioreactor. Microb Cell Fact 22: 153.
  • Hoang MD, Riessner S, Oropeza Vargas JE, von den Eichen N, Heins AL (2023): Influence of varying pre-culture conditions on the level of population heterogeneity in batch cultures with an Escherichia coli triple reporter strain. Microorganisms 11: 1763.
  • Hoang MD, Doan DT, Schmidt M, Kranz H, Kremling A, Heins A-L (2023): Application of an Escherichia coli triple reporter strain for at-line monitoring of single-cell physiology during L-phenylalanine production. Eng Life Sci 23: e2100162.
  • Doan DT, Hoang MD, Heins A-L, Kremling A (2022): Applications of coarse-grained models in metabolic engineering. Front Mol Biosc 9: 806213.
  • Heins A-L, Hoang MD, Weuster-Botz D (2022): Advances in automated real-time flow cytometry for monitoring of bioreactor processes. Eng Life Sci, 22: 260-278.
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