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Technical University of Munich

Synthetic co-cultivation of C. carboxidivorans with C. kluyveri for syngas conversion

Miriam Bäumler, doctoral thesis Technical University of Munich, 2024

The establishment of a synthetic co-culture of Clostridium carboxidivorans and Clostridium kluyveri allowed the autotrophic conversion of carbon monoxide to longer-chain alcohols. 1-Butanol and 1-hexanol concentrations could be increased several fold compared to a pure culture of C. carboxidivorans. Transfer of the process to a continuously operated stirred-tank bioreactor cascade significantly increased the space-time yields for ethanol (factor 5.3), 1-butanol (factor 2.7), and 1-hexanol (factor 1.9).

Publications
  • Bäumler M, Burgmaier V, Herrmann F, Mentges J, Schneider M, Ehrenreich A, Liebl W, Weuster-Botz D (2023): Continuous production of ethanol, 1-butanol and 1-hexanol from CO with a synthetic co-culture of Clostridia applying a cascade of stirred-tank bioreactors. Microorganisms 11: 1003.
  • Herzog J, Mook A, Guhl L, Bäumler M, Beck MH, Weuster-Botz D, Bengelsdorf FR, Zeng A-P (2023): Novel synthetic co-culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid. Eng Life Sci 23: e2100169.
  • Mittermeier F, Bäumler M, Arulrajah P, García Lima JJ, Hauke S, Stock A, Weuster-Botz D (2023): Artificial microbial consortia for bioproduction processes. Eng Life Sci 23: e2100152.
  • Bäumler M, Schneider M, Ehrenreich A, Liebl W, Weuster-Botz D (2022): Synthetic co-culture of autotrophic Clostridium carboxidivorans with chain elongating Clostridium kluyveri monitored by flow cytometry. Microb Biotechnol 15: 1471–1485.
  • Schneider M, Bäumler M, Lee NM, Weuster-Botz D, Ehrenreich A, Liebl W (2021): Monitoring co-cultures of Clostridium carboxidivorans and Clostridium kluyveri by fluorescence in situ hybridization with specific 23S rRNA oligonucleotide probes. System Appl Microbiol 44: 126271.
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