Production of halophilc enzymes using Haloferax volcanii in a stirred-tank bioreactor

Eva Strillinger, Doctoral Thesis Technische Universität München, 2016

Production of enzymes originating from extremophilic habitats is often not possible when conventional expression systems are used. Therefore, biofilm formation was deactivated genetically in the halophilic archaeon Haloferax volcanii. This allowed the design of a scalable batch process resulting in high cell densities in a stirred tank bioreactor. For the first time Haloferax volcanii was used for recombinant production and characterization of novel oxidoreductases out of the Deep Red Sea Brine Pools.

Publications

• Groetzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi I, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll, M, Eppinger J, Arold ST (2018): Identification and experimental characterization of an extremophilic brine pool alcohol dehydrogenase from single amplified genomes. ACS Chem Biol 13: 161-170.
• Strillinger E, Grötzinger SW, Allers T, Groll M, Eppinger J, Weuster-Botz D (2016): Production of halophilic proteins with Haloferax volcanii H1895 in a stirred tank bioreactor. App Microbiol Biotechnol 100: 1183–1195.