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Technical University of Munich

Reaction Engineering Optimisation of Parallel Reactors for Controlled Bioprocesses

Andreas Kusterer, doctoral thesis Technische Universität München, 2007

One of the most sophisticated challenges in the development and optimisation of biotechnological production processes is the efficient extraction of relevant process data. By advancing the design of a magnetic-inductively driven stirred-tank bioreactor system on a millilitre-scale and the application of an optical sensor technology for the measurement of pH and dissolved oxygen, an individual pH-control of 48 parallel bioreactors was achieved. This tool for parallel bioprocess development under controlled technical conditions was validated with exemplary bioprocesses making use of Escherichia coli and Saccharomyces cerevisiae.

Publications

  • Kusterer A, Krause C, Kaufmann K, Arnold M, Weuster-Botz D (2008): Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations. Bioprocess Biosyst Eng 31: 207-215.
  • Weuster-Botz D, Puskeiler R, Kusterer A, Kaufmann K, John GT, Arnold M (2005): Methods and milliliter scale devices for high-throughput bioprocess design. Bioprocess Biosyst Eng 28: 109-119.
  • Puskeiler R, Kusterer A, John GT, Weuster-Botz D (2005): Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli. Biotechnol Appl Biochem 42: 227-235.
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